Inoveli yeCoronavirus (2019-nCoV) yeNucleic Acid Test Kit
Iinkcukacha zeMveliso:
Uvavanyo lwe-Innovita® 2019-nCoV IgM/IgG lwenzelwe ukuxilongwa kunye nokuhlolwa kwe-epidemiological yezifo ezibangelwa yinoveli coronavirus (2019-nCoV).I-ORF1ab kunye ne-N gene ka-2019-nCoV zichongiwe ngokusemgangathweni kwi-throat swabs kunye neesampulu ze-alveolar lavage fluid eziqokelelwe kwiimeko ezikrokrelekayo zenyumoniya, izigulana ezineemeko ezikrokrelekayo, abanye abafuna ukuxilonga.
Iziphumo zovavanyo zale khithi zezereferensi yezonyango kuphela.Kunconywa ukuba kuqhutywe uhlalutyo olubanzi lwemeko esekelwe kwimibonakaliso yekliniki yesigulane kunye nezinye iimvavanyo zebhubhoratri.
Umgaqo:
Le khithi isebenzisa inyathelo elinye lokuguqulela umva le-polymerase chain reaction (RT-I-PCR) itekhnoloji yokufumanisa ukujolisa kwinoveli yekoronavirus (2019-nCoV) i-ORF1ab gene, i-N gene, kunye nolandelelwano lwangaphakathi lwereferensi yomntu.Iiprimers ezithile kunye ne-taqman probes ziyilwe kwimimandla egciniweyo.
Ukuqulunqwa:
| Ukuqamba | 48 iimvavanyo / ikhithi |
| Umxube wokusabela A | 792μL×1Tube |
| Umxube wokusabela B | 168μL×1Tube |
| Ulawulo oluhle | 50μL×1Tube |
| Ulawulo olubi | 50μL ×1Tube |
Qaphela: 1. Iibhetshi ezahlukeneyo ze-reagents akufanele zixutywe.
2. Ulawulo oluhle kunye nolawulo olufanelekileyo akufuneki lukhutshwe
Inkqubo yoVavanyo:
1. Utsalo lwe-Nucleic acid:
Izixhobo zokurhweba zeRNA ziyafumaneka, ukutsalwa kwentsimbi yemagnethi kunye nokutsalwa kwekholamu ye-spin kuyacetyiswa kule khithi.
2. Lungiselela uMxube weRection:
● Phuma kwi-2019-nCoV reaction Mix A/B kwaye ugcine kwiqondo lobushushu begumbi de ube ungagodoli;
● Thatha amacandelo ahambelanayo (I-Reaction Mix A 16.5μL/T, i-Reaction Mix B 3.5μL/T) kwaye udibanise, kwaye ke aliquot ukuphendula kwe-PCR nganye nge-20μL / ityhubhu;
● Yongeza i-5μL ye-template ye-RNA okanye ulawulo olubi okanye ulawulo oluhle, emva koko uvale i-tube cap;
● Beka i-reaction ityhubhu kwisixhobo se-PCR se-fluorescence, kwaye usete ulawulo olubi / olulungileyo kunye neeparamitha zesampulu ze-RT-PCR ngokwemiyalelo yokusebenza kwesixhobo.
● Rekhoda i-odolo yokubekwa kweSampuli
Iiprothokholi ze-3.RT-PCR:
Iisetingi ezicetyiswayo:
| Umjikelo | Ixesha | Ubushushu(℃） | |
| 1 | 1 | 10min | 25 |
| 2 | 1 | 10min | 50 |
| 3 | 1 | 10min | 95 |
| 4 | 45 | 10s | 95 |
| 35s | 55 |