2019-nCoV Uvavanyo lwe-Antibody engathathi hlangothi (QDIC)
Iinkcukacha zeMveliso:
Uvavanyo lwe-Innovita® 2019-nCoV IgM/IgG lwenzelwe ukufunyaniswa kobungakanani bokungathathi hlangothi kwe-antibody kwinoveli coronavirus (2019-nCoV) kwiserum yomntu, iplasma okanye igazi elipheleleyo (igazi leminwe okanye igazi elipheleleyo le-venous).
I-2019-nCoV ibandakanya iiprotheyini ezine eziphambili: iprotheni ye-S, i-E protein, i-M protein kunye ne-N protein.Ummandla we-RBD we-S protein unokubophelela kwi-cell surface receptor ACE2.Uphononongo lubonise ukuba iisampulu zabantu abaye baphola kusulelo lwenoveli ye-coronavirus banethemba lokunciphisa i-antibody.Ukufunyaniswa kwe-antibody engathathi hlangothi kungasetyenziselwa ukuvavanya ukuxilongwa kosulelo lwentsholongwane kunye novavanyo lwesiphumo emva kogonyo.
Umgaqo:
Ikhithi yi-quantum dot immunofluorescence chromatography assay ukufumanisa i-2019-nCoV RBD ekhethekileyo ye-IgG engathathi hlangothi kwi-serum yomntu, iplasma okanye igazi elipheleleyo (igazi leminwe kunye negazi elipheleleyo le-venous).Emva kokuba i-specimen isetyenziswe kwi-specimen kakuhle, ukuba i-concentration of neutralizing antibodies iphezulu kunomda ophantsi wokufumanisa, i-antibodies ye-RBD ekhethekileyo ye-IgG iya kusabela kunye nenxalenye okanye yonke i-antigen ye-RBD ebhalwe nge-quantum dot microspheres ukwenza i-immune compound.Emva koko i-immune compound iya kufuduka ecaleni kwe-nitrocellulose membrane.Xa befikelela kwindawo yokuvavanya (umgca we-T), i-compound iya kusabela nge-mouse anti-human IgG (γ chain) efakwe kwi-membrane ye-nitrocellulose kwaye yenza umgca we-fluorescent.Funda ixabiso lesignali ye-fluorescence kunye ne-fluorescence immunoassay analyzer.Ixabiso lesignali lilingana nomxholo we-neutralizing antibodies kwi-specimen.
Ingaba i-specimen iqulethe i-RBD ekhethekileyo ye-neutralizing antibodies okanye cha, umgca wokulawula kufuneka uhlale ubonakala kwiwindow yesiphumo ukuba inkqubo yokuvavanya iqhutywe ngokufanelekileyo kwaye i-reagent isebenza njengoko ijoliswe kuyo.Xa inkukhu i-antibody ye-IgY ebhalwe nge-quantum dot microspheres ifudukela kumgca wokulawula (C line), iyakubanjwa yibhokhwe ye-anti-chicken IgY antibody efakwe ngaphambili kumgca we-C, kwaye umgca we-fluorescent wenziwa.Umgca wolawulo (umgca weC) usetyenziswa njengolawulo lwenkqubo.
Ukuqulunqwa:
| Ukuqamba | Isixa | Inkcazo |
| IFU | 1 | / |
| Ikhasethi yovavanyo | 20 | Isingxobo ngasinye sefoil esitywiniweyo esinesixhobo esinye sovavanyo kunye ne-desiccant enye |
| Isampulu sixubile | 3mL*1 ingqayi | I-20mM i-PBS, i-Sodium Casein, i-ProClin 300 |
| Micropipette | 20 | I-Micropipette ene-20μL yomgca wokumakisha |
| I-Lancet | 20 | / |
| Iphedi yotywala | 20 | / |
Inkqubo yoVavanyo:
● Ukuqokelelwa kwegazi ngeminwe
● Funda umphumo kunye ne-fluorescence analyzer
