Rotavirus/Adenovirus/Norovirus Ag Test
Usetyenziso olucetywayo
Ikiti yenzelwe ukufumanisa ngokuthe ngqo kunye nomgangatho weqela le-Antigens ye-rotavirus, i-adenovirus antigens 40 kunye ne-41, i-norovirus (GI) kunye ne-norovirus (GII) ye-antigens kwi-specimens yendle yabantu.
Iziphumo zovavanyo oluqinisekileyo zifuna uqinisekiso olongezelelweyo.Iziphumo zovavanyo olubi alukhupheli phandle ukuba kungenzeka usulelo.
Iziphumo zovavanyo zale khithi zezereferensi yezonyango kuphela.Kunconywa ukuba kuqhutywe uhlalutyo olubanzi lwemeko esekelwe kwimibonakaliso yekliniki yesigulane kunye nezinye iimvavanyo zebhubhoratri.
Isishwankathelo
I-Rotavirus (RV)yintsholongwane ebalulekileyo ebangela urhudo lwentsholongwane kunye ne-enteritis kwiintsana kunye nabantwana abancinci kwihlabathi jikelele.Incopho yezehlo isekwindla, ekwabizwa ngokuba "yi-autumn diarrhea yeentsana kunye nabantwana abancinci".Iziganeko zezifo zentsholongwane kwiintsana phakathi kweenyanga kunye neminyaka emi-2 ubudala ziphakamileyo ukuya kuma-62%, kwaye ixesha lokufukamela liyi-1 ukuya kwiintsuku ze-7, ngokuqhelekileyo ngaphantsi kweeyure ezingama-48, ezibonakaliswe ngohudo olunzima kunye nokuphelelwa ngamanzi emzimbeni.Emva kokuhlasela umzimba womntu, iphinda-phinda kwiiseli ze-epithelial ezimbi zamathumbu amancinci kwaye ikhutshwe ngobuninzi obukhulu ngeelindle.
I-Adenovirus (ADV)yintsholongwane ye-DNA enemisonto ephindwe kabini enobubanzi obuyi-70-90nm.Yintsholongwane elinganayo ye-icosahedral engenamvulophu.Amasuntswana entsholongwane enziwe ikakhulu ngamaqokobhe eprotheyini kunye neDNA enemisonto ephindwe kabini.Uhlobo lwe-adenovirus ye-Enteric 40 kunye nohlobo lwe-41 ye-subgroup F yintsholongwane ebalulekileyo yesifo sohudo lwentsholongwane ebantwini, ikakhulu sichaphazela iintsana kunye nabantwana abancinci (ngaphantsi kweminyaka emi-4 ubudala).Ixesha lokufukamela limalunga neentsuku ezi-3 ukuya kwezili-10.Iphindaphinda kwiiseli zamathumbu kwaye ikhutshwe kwi-feces iintsuku ezili-10.Ukubonakaliswa kweklinikhi kubuhlungu besisu, isifo sohudo, indle yamanzi, ehamba nomkhuhlane kunye nokuhlanza.
Norovirus (NoV)yeyosapho lwe-caliciviridae kwaye inamaqhekeza angama-20-hedral anobubanzi obuyi-27-35 nm kwaye ayinamvulophu.I-Norovirus yenye ye-pathogens eziphambili ezibangela i-non-bacterial acute gastroenteritis okwangoku.Le ntsholongwane iyosulela kakhulu kwaye isasazeka ngamanzi angcolisekileyo, ukutya, ukuhanjiswa kunye ne-aerosol eyenziwe zizingcolisi.I-Norovirus yintsholongwane yesibini ebangela isifo sohudo kubantwana, kwaye iphuma kwiindawo ezixineneyo.Ii-Noroviruses zahlulwe kakhulu kwiigenomes ezintlanu (GI, GII, GIII, GIV kunye ne-GV), kwaye ezona zifo ziphambili zosulelo lwabantu yi-GI, i-GII kunye ne-GIV, phakathi kwazo i-GII genome zezona zixhaphakileyo zentsholongwane emhlabeni jikelele.Iindlela zokuxilonga zeklinikhi okanye zaselabhoratri zosulelo lwe-norovirus ikakhulu lubandakanya i-electron microscopy, i-molecular biology kunye nokufumanisa i-immunological.
Ukuqamba
Ukuqokelelwa komfanekiso kunye nokuPhathwa
1. Qokelela isampuli yelindle kwindawo ecocekileyo, neyomileyo.
2. Vula isixhobo sokuqokelela ilindle ngokukhulula umphezulu kwaye usebenzise ifotsholo yokuqokelela ngokungacwangciswanga
3. gqobhoza isampuli yelindle kwiindawo ezi-2 ~ 5 ezahlukeneyo zokuqokelela malunga ne-100mg yelindle eliqinileyo (elilingana ne-1/2 ye-ertyisi) okanye i-100μL yelindle elingamanzi.Musa ukukhupha isampuli yelindle njengoko oku kunokukhokelela kwisiphumo sovavanyo esingasebenziyo.
4. Qinisekisa ukuba umfuziselo welindle ukwimijelo yokuqokelela ifotsholo kuphela.Umlinganiselo welindle eligqithileyo unokukhokelela kwisiphumo sovavanyo esingasebenziyo.
5. Khupha kwaye uqinise ikepusi kwisixhobo sokuqokelela isampuli.
6. Vuthulula ngamandla isixhobo sokuqokelela ilindle.
Inkqubo yoVavanyo
1. Yiza nomzekelo kunye nezixhobo zovavanyo kwiqondo lobushushu begumbi ukuba ifriji okanye ifriziwe.
2. Xa sele ulungele ukuqalisa uvavanyo, vula isingxobo esivaliweyo ngokukrazula inotshi.Susa uvavanyo kwisingxobo.
3. Beka isixhobo sovavanyo kwindawo ecocekileyo nesicaba.
4. Misa isixhobo sokuqokelela ilindle sime nkqo kwaye ujije isivalo se-dispenser.
5. Ukubamba isixhobo sokuqokelela ilindle ngokuthe nkqo, sebenzisa i-80μL (malunga namathontsi ama-2) yesisombululo kwiqula lomfuziselo wesixhobo sovavanyo.Musa ukulayisha kakhulu isampuli.
6. Funda iziphumo zovavanyo kwimizuzu eli-15.Musa ukufunda umphumo emva kwemizuzu eyi-15.
Utoliko lweziphumo
1. Okuhle:Ubukho bemigca emibini ebomvu-mfusa (i-T kunye no-C) ngaphakathi kwefestile yesiphumo ibonisa i-antigen ye-RV/ADV/NoV.
2. Engalunganga:Umgca omnye obomvu-omfusa ovela kumgca wokulawula (C) ubonisa umphumo ongalunganga.
3. Ayisebenzi:Ukuba umgca wolawulo (C) uhluleka ukuvela, kungakhathaliseki ukuba umgca we-T ubonakala okanye awubonakali, uvavanyo aluvumelekile.Phonononga inkqubo kwaye uluphinde uvavanyo ngesixhobo esitsha sovavanyo.
