Iimveliso

Iinkcukacha zeMveliso:

Uvavanyo lwe-Innovita® 2019-nCoV IgM/IgG lwenzelwe ukufunyaniswa kwesiqingatha sobungakanani bokungathinteli amajoni omzimba kwinoveli coronavirus (2019-nCoV) kwiserum yomntu, iplasma okanye iisampulu zegazi lonke.
I-2019-nCoV ibandakanya iiprotheyini ezine eziphambili: iprotheni ye-S, i-E protein, i-M protein kunye ne-N protein.Ummandla we-RBD we-S protein unokubophelela kwi-cell surface receptor ACE2.I-antibody engathathi hlangothi ibhekisa kwisakhono sokuzibophelela kunye ne-pathogen, kwaye emva koko ivale i-pathogen ukuba ingene emzimbeni ukuze ibangele usulelo.Ukufunyaniswa kwe-antibody engathathi hlangothi kungasetyenziselwa ukuvavanya ukuxilongwa kosulelo lwentsholongwane.

Umgaqo:

Ikhithi lukhuphiswano lwe-colloid yegolide ye-immunochromatography yokufumanisa ii-antibodies eziya kuthi ga kwi-2019-nCoV kwiserum yomntu, iplasma okanye iisampulu zegazi elipheleleyo.Emva kokuba i-specimen isetyenziswe kwi-specimen kakuhle, ukuba i-anti-neutralizing antibodies ikhona kwi-specimen, i-anti-neutralizing antibodies iya kusabela ngegolide ye-colloidal ebhalwe i-RBD antigen ukwenza i-immune complex, kwaye indawo yokungathathi hlangothi ye-antigen ye-RBD iya kuvalwa.Emva koko i-immune complex kunye nelebhile ye-antigen ye-RBD ngaphandle kokuzibophelela kwi-antibody engathathi hlangothi ifuduka ecaleni kwe-nitrocellulose membrane.Xa befikelela kwindawo yovavanyo (umgca we-T), i-antigen ebhalwe nge-RBD ngaphandle kokuzibophelela kwi-neutraizing antibodies iya kusabela nge-antigen ye-ACE2 efakwe kwi-nitrocellulose membrane kwaye yenze umgca omfusa-obomvu.Xa i-concentration of neutralizing antibodies iphezulu kunomlinganiselo ophantsi wokufumanisa, umgca obomvu-obomvu ulula kunomgca wokulawula (umgca we-C) okanye akukho mgca obomvu obomvu owenziweyo, umphumo ulungile.Xa i-concentration ye-neutralizing antibodies iphantsi kunomda ophantsi wokufumanisa okanye akukho zi-antibodies ze-neutralizing kwi-specimen, umgca obomvu-obomvu ubumnyama kunomgca wokulawula, umphumo ubi.
Nokuba lo mzekelo uqulathe i-2019-nCoV neutralizing antibodies, xa i-antibody ebhalwe ngegolide ye-colloidal ye-IgY ifudukela kumgca wolawulo (C line), iya kubanjwa yibhokhwe ye-anti-chicken IgY antibody efakwe ngaphambili kumgca wolawulo (C). umgca), umgca omfusa-obomvu wenziwa.Umgca wolawulo (umgca weC) usetyenziswa njengolawulo lwenkqubo.Imigca yolawulo kufuneka ihlale ivela kwiifestile zesiphumo ukuba inkqubo yovavanyo yenziwa ngokufanelekileyo kwaye ii-reagents zisebenza njengoko kucetywayo.

Ukuqulunqwa:

Inkqubo yoVavanyo:

1. Khulula isingxobo sefoyile yealuminiyam uze ukhuphe ikhasethi yovavanyo.
2. Faka i-40μL ye-serum/i-plasma ye-specimen okanye i-60μL yonke isampuli yegazi kumzekelo kakuhle.
3. Faka i-40μL (amathontsi ama-2) isampulu ediluyi kwisampulu kakuhle.
4. Yibeke kwindawo yokushisa (15 ℃ ~ 30 ℃) imizuzu eyi-15-20, kwaye ufunde umphumo.

Iziphumo Utoliko:

1. I-Positive: Xa umbala womgca we-T ukhaphukhaphu kunowomgca we-C okanye xa kungekho mgca u-T, ubonisa ukuba ulungile kwi-neutralizing antibodies.
2. Okubi: Xa umbala womgca we-T umnyama kuno okanye ulingana nomgca we-C, ubonisa okungalunganga kwi-neutralizing antibodies.
3. Okungavumelekanga: Xa umgca we-C ungaphumeleli, kungakhathaliseki ukuba umgca we-T ubonakala okanye awubonakali, uvavanyo aluvumelekanga.Phinda uvavanyo ngovavanyo olutsha.